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Here, we show that the peptidase da1 physically interacts with and cleaves the wus protein, leading to its destabilization We generated da1_dar1a_dar1b triple crispr maize mutants and bb1_bb2 double mutants. Furthermore, our results reveal that cytokinin signaling represses the.
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Genetic analyses support that da1 acts through wox5 to regulate root stem cell function Here, we characterized the da1, dar1 and bb gene families in maize and analyzed whether perturbation of these genes regulates organ size similar to what was observed in arabidopsis We further demonstrate that cytokinin (ck) signaling induces the accumulation of da1 protein, thereby decreasing the abundance of wox5 protein in the root.
We propose that da1 peptidase activity regulates the duration of cell proliferation and the transition to endoreduplication and differentiation during organ formation in plants by coordinating the destabilization of regulatory proteins.
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